# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> RUN SCRIPT <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
-my $t0 = gettimeofday();
-
run_script( $script );
-my $t1 = gettimeofday();
-
-print STDERR "Program: $script" . ( " " x ( 25 - length( $script ) ) ) . sprintf( "Run time: %.4f\n", ( $t1 - $t0 ) );
-
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> SUBROUTINES <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
# Returns nothing.
- my ( $options, $in, $out );
+ my ( $t0, $t1, $options, $in, $out );
+
+ $t0 = gettimeofday();
$options = get_options( $script );
close $in if defined $in;
close $out;
+
+ $t1 = gettimeofday();
+
+ print STDERR "Program: $script" . ( " " x ( 25 - length( $script ) ) ) . sprintf( "Run time: %.4f\n", ( $t1 - $t0 ) ) if $options->{ 'verbose' };
}
@options = qw(
adaptor|a=s
mismatches|m=s
- no_remove|n
+ remove|r=s
offset|o=s
);
}
{
Maasha::Common::error( qq(Argument to --$opt must be octal or decimal - not "$options{ $opt }") );
}
+ elsif ( $opt eq "remove" and $script eq "remove_adaptor" and $options{ $opt } !~ /before|after|skip/ )
+ {
+ Maasha::Common::error( qq(Argument to --$opt must be before, after, or skip - not "$options{ $opt }") );
+ }
}
Maasha::Common::error( qq(no --database specified) ) if $script eq "create_blast_db" and not $options{ "database" };
{
# Martin A. Hansen, Juli 2008.
- # Read fixedStep wiggle format from stream or file.
+ # Read fixedstep wiggle format from stream or file.
my ( $in, # handle to in stream
$out, # handle to out stream
$phastcons_file = Maasha::Config::genome_phastcons( $options->{ "genome" } );
$phastcons_index = Maasha::Config::genome_phastcons_index( $options->{ "genome" } );
- $index = Maasha::UCSC::phastcons_index_retrieve( $phastcons_index );
+ $index = Maasha::UCSC::fixedstep_index_retrieve( $phastcons_index );
$fh_phastcons = Maasha::Common::read_open( $phastcons_file );
if ( defined $options->{ "chr" } and defined $options->{ "beg" } and ( defined $options->{ "end" } or defined $options->{ "len" } ) )
$options->{ "end" } = $options->{ "beg" } + $options->{ "len" } - 1;
}
- $scores = Maasha::UCSC::phastcons_index_lookup( $index, $fh_phastcons, $options->{ "chr" }, $options->{ "beg" }, $options->{ "end" }, $options->{ "flank" } );
+ $scores = Maasha::UCSC::fixedstep_index_lookup( $index, $fh_phastcons, $options->{ "chr" }, $options->{ "beg" }, $options->{ "end" }, $options->{ "flank" } );
$record->{ "CHR" } = $options->{ "chr" };
$record->{ "CHR_BEG" } = $options->{ "beg" } - $options->{ "flank" };
my ( $record, $adaptor, $seq, $adaptor_len, $seq_len, $offset, $max_match, $max_mismatch, $pos );
+ $options->{ "remove" } ||= "after";
+
$max_mismatch = $options->{ "mismatches" } || 0;
$offset = $options->{ "offset" };
$record->{ "ADAPTOR_POS" } = $pos;
- if ( $pos >= 0 and not $options->{ "no_remove" } )
+ if ( $pos >= 0 and $options->{ "remove" } ne "skip" )
{
- $record->{ "SEQ" } = substr $record->{ "SEQ" }, 0, $pos;
- $record->{ "SEQ_LEN" } = $pos;
+ if ( $options->{ "remove" } eq "after" )
+ {
+ $record->{ "SEQ" } = substr $record->{ "SEQ" }, 0, $pos;
+ $record->{ "SEQ_LEN" } = $pos;
+ }
+ else
+ {
+ $record->{ "SEQ" } = substr $record->{ "SEQ" }, $pos + $adaptor_len;
+ $record->{ "SEQ_LEN" } = length $record->{ "SEQ" };
+ }
}
put_record( $record, $out );
while ( $record = get_record( $in ) )
{
- $data_hash{ $record->{ $options->{ "key" } } }++ if $record->{ $options->{ "key" } };
+ $data_hash{ $record->{ $options->{ "key" } } }++ if defined $record->{ $options->{ "key" } };
put_record( $record, $out ) if not $options->{ "no_stream" };
}
while ( $record = get_record( $in ) )
{
- $data_hash{ $record->{ $options->{ "key" } } }++ if $record->{ $options->{ "key" } };
+ $data_hash{ $record->{ $options->{ "key" } } }++ if defined $record->{ $options->{ "key" } };
put_record( $record, $out ) if not $options->{ "no_stream" };
}
{
# Martin A. Hansen, July 2008.
- # Cleans out any unused temporary files and direcotries in BP_TMP.
+ # Cleans out any unused temporary files and directories in BP_TMP.
# Returns nothing.
elsif ( $pid == $curr_pid )
{
# print STDERR "Removing current dir: $dir\n";
- Maasha::Common::dir_remove( $dir );
+ # Maasha::Common::dir_remove( $dir );
}
}
}