* vcfutils.pl: fixed a typo in help message

[samtools.git] / bcftools / vcfutils.pl

diff --git a/bcftools/vcfutils.pl b/bcftools/vcfutils.pl
index 371e8d84181dd5bc55aa5cfa0777ae7535ff4f69..eed8766464e9b63e08268a97b146d3464705a042 100755 (executable)
--- a/bcftools/vcfutils.pl
+++ b/bcftools/vcfutils.pl
@@ -14,7 +14,7 @@ sub main {
   &usage if (@ARGV < 1);
   my $command = shift(@ARGV);
   my %func = (subsam=>\&subsam, listsam=>\&listsam, fillac=>\&fillac, qstats=>\&qstats, varFilter=>\&varFilter,
-                         hapmap2vcf=>\&hapmap2vcf, ucscsnp2vcf=>\&ucscsnp2vcf);
+                         hapmap2vcf=>\&hapmap2vcf, ucscsnp2vcf=>\&ucscsnp2vcf, filter4vcf=>\&filter4vcf);
   die("Unknown command \"$command\".\n") if (!defined($func{$command}));
   &{$func{$command}};
 }
@@ -70,12 +70,21 @@ sub fillac {
          print;
        } else {
          my @t = split;
-         my @c;
+         my @c = (0);
          my $n = 0;
-         $c[1] = 0;
+         my $s = -1;
+         @_ = split(":", $t[8]);
+         for (0 .. $#_) {
+               if ($_[$_] eq 'GT') { $s = $_; last; }
+         }
+         if ($s < 0) {
+               print join("\t", @t), "\n";
+               next;
+         }
          for (9 .. $#t) {
-               if ($t[$_] =~ /^(\d+).(\d+)/) {
-                 ++$c[$1]; ++$c[$2];
+               if ($t[$_] =~ /^0,0,0/) {
+               } elsif ($t[$_] =~ /^([^\s:]+:){$s}(\d+).(\d+)/) {
+                 ++$c[$2]; ++$c[$3];
                  $n += 2;
                }
          }
@@ -307,6 +316,42 @@ sub varFilter_aux {
   }
 }
 
+sub filter4vcf {
+  my %opts = (d=>3, D=>2000, 1=>1e-4, 2=>1e-100, 3=>0, 4=>1e-4, Q=>10, q=>3);
+  getopts('d:D:1:2:3:4:Q:q:', \%opts);
+  die(qq/
+Usage:   vcfutils.pl filter4vcf [options] <in.vcf>
+
+Options: -d INT     min total depth (given DP or DP4) [$opts{d}]
+         -D INT     max total depth [$opts{D}]
+         -q INT     min SNP quality [$opts{q}]
+         -Q INT     min RMS mapQ (given MQ) [$opts{Q}]
+         -1 FLOAT   min P-value for strand bias (given PV4) [$opts{1}]
+         -2 FLOAT   min P-value for baseQ bias [$opts{2}]
+         -3 FLOAT   min P-value for mapQ bias [$opts{3}]
+         -4 FLOAT   min P-value for end distance bias [$opts{4}]\n
+/) if (@ARGV == 0 && -t STDIN);
+
+  my %ts = (AG=>1, GA=>1, CT=>1, TC=>1);
+
+  my @n = (0, 0);
+  while (<>) {
+       next if (/^#/);
+       next if (/PV4=([^,]+),([^,]+),([^,]+),([^,;\t]+)/ && ($1<$opts{1} || $2<$opts{2} || $3<$opts{3} || $4<$opts{4}));
+       my $depth = -1;
+       $depth = $1 if (/DP=(\d+)/);
+       $depth = $1+$2+$3+$4 if (/DP4=(\d+),(\d+),(\d+),(\d+)/);
+       next if ($depth > 0 && ($depth < $opts{d} || $depth > $opts{D}));
+       next if (/MQ=(\d+)/ && $1 < $opts{Q});
+       my @t = split;
+       next if ($t[5] >= 0 && $t[5] < $opts{q});
+       ++$n[0];
+       my @s = split(',', $t[4]);
+       ++$n[1] if ($ts{$t[3].$s[0]});
+       print;
+  }
+}
+
 sub ucscsnp2vcf {
   die("Usage: vcfutils.pl <in.ucsc.snp>\n") if (@ARGV == 0 && -t STDIN);
   print "##fileformat=VCFv4.0\n";
@@ -400,6 +445,7 @@ Command: subsam       get a subset of samples
          fillac       fill the allele count field
          qstats       SNP stats stratified by QUAL
          varFilter    filtering short variants
+         filter4vcf   filtering VCFs produced by samtools+bcftools
          hapmap2vcf   convert the hapmap format to VCF
          ucscsnp2vcf  convert UCSC SNP SQL dump to VCF
 \n/);