KSORT_INIT(heap, heap1_t, heap_lt)
+static void swap_header_text(bam_header_t *h1, bam_header_t *h2)
+{
+ int tempi;
+ char *temps;
+ tempi = h1->l_text, h1->l_text = h2->l_text, h2->l_text = tempi;
+ temps = h1->text, h1->text = h2->text, h2->text = temps;
+}
+
/*!
@abstract Merge multiple sorted BAM.
@param is_by_qname whether to sort by query name
@param out output BAM file name
+ @param headers name of SAM file from which to copy '@' header lines,
+ or NULL to copy them from the first file to be merged
@param n number of files to be merged
@param fn names of files to be merged
@discussion Padding information may NOT correctly maintained. This
function is NOT thread safe.
*/
-void bam_merge_core(int by_qname, const char *out, int n, char * const *fn)
+void bam_merge_core(int by_qname, const char *out, const char *headers, int n, char * const *fn)
{
bamFile fpout, *fp;
heap1_t *heap;
bam_header_t *hout = 0;
+ bam_header_t *hheaders = NULL;
int i, j;
+ if (headers) {
+ tamFile fpheaders = sam_open(headers);
+ if (fpheaders == 0) {
+ fprintf(stderr, "[bam_merge_core] Cannot open file `%s'. Continue anyway.\n", headers);
+ } else {
+ hheaders = sam_header_read(fpheaders);
+ sam_close(fpheaders);
+ }
+ }
+
g_is_by_qname = by_qname;
fp = (bamFile*)calloc(n, sizeof(bamFile));
heap = (heap1_t*)calloc(n, sizeof(heap1_t));
for (i = 0; i != n; ++i) {
heap1_t *h;
bam_header_t *hin;
- assert(fp[i] = bam_open(fn[i], "r"));
+ fp[i] = bam_open(fn[i], "r");
+ if (fp[i] == 0) {
+ int j;
+ fprintf(stderr, "[bam_merge_core] fail to open file %s\n", fn[i]);
+ for (j = 0; j < i; ++j) bam_close(fp[j]);
+ free(fp); free(heap);
+ // FIXME: possible memory leak
+ return;
+ }
hin = bam_header_read(fp[i]);
- if (i == 0) hout = hin;
- else { // validate multiple baf
+ if (i == 0) { // the first SAM
+ hout = hin;
+ if (hheaders) {
+ // If the text headers to be swapped in include any @SQ headers,
+ // check that they are consistent with the existing binary list
+ // of reference information.
+ if (hheaders->n_targets > 0) {
+ if (hout->n_targets != hheaders->n_targets)
+ fprintf(stderr, "[bam_merge_core] number of @SQ headers in `%s' differs from number of target sequences", headers);
+ for (j = 0; j < hout->n_targets; ++j)
+ if (strcmp(hout->target_name[j], hheaders->target_name[j]) != 0)
+ fprintf(stderr, "[bam_merge_core] @SQ header '%s' in '%s' differs from target sequence", hheaders->target_name[j], headers);
+ }
+ swap_header_text(hout, hheaders);
+ bam_header_destroy(hheaders);
+ hheaders = NULL;
+ }
+ } else { // validate multiple baf
if (hout->n_targets != hin->n_targets) {
fprintf(stderr, "[bam_merge_core] file '%s' has different number of target sequences. Abort!\n", fn[i]);
exit(1);
hout->target_name[j], hin->target_name[j], fn[i]);
exit(1);
}
- if (hout->target_len[j] != hin->target_len[j])
- fprintf(stderr, "[bam_merge_core] different target sequence length: %d != %d in file '%s'. Continue.\n",
- hout->target_len[j], hin->target_len[j], fn[i]);
}
bam_header_destroy(hin);
}
int bam_merge(int argc, char *argv[])
{
int c, is_by_qname = 0;
- while ((c = getopt(argc, argv, "n")) >= 0) {
+ char *fn_headers = NULL;
+
+ while ((c = getopt(argc, argv, "h:n")) >= 0) {
switch (c) {
+ case 'h': fn_headers = strdup(optarg); break;
case 'n': is_by_qname = 1; break;
}
}
if (optind + 2 >= argc) {
- fprintf(stderr, "Usage: samtools merge [-n] <out.bam> <in1.bam> <in2.bam> [...]\n");
+ fprintf(stderr, "\n");
+ fprintf(stderr, "Usage: samtools merge [-n] [-h inh.sam] <out.bam> <in1.bam> <in2.bam> [...]\n\n");
+ fprintf(stderr, "Options: -n sort by read names\n");
+ fprintf(stderr, " -h FILE copy the header in FILE to <out.bam> [in1.bam]\n\n");
+ fprintf(stderr, "Note: Samtools' merge does not reconstruct the @RG dictionary in the header. Users\n");
+ fprintf(stderr, " must provide the correct header with -h, or uses Picard which properly maintains\n");
+ fprintf(stderr, " the header dictionary in merging.\n\n");
return 1;
}
- bam_merge_core(is_by_qname, argv[optind], argc - optind - 1, argv + optind + 1);
+ bam_merge_core(is_by_qname, argv[optind], fn_headers, argc - optind - 1, argv + optind + 1);
+ free(fn_headers);
return 0;
}
}
KSORT_INIT(sort, bam1_p, bam1_lt)
-static void sort_blocks(int n, int k, bam1_p *buf, const char *prefix, const bam_header_t *h)
+static void sort_blocks(int n, int k, bam1_p *buf, const char *prefix, const bam_header_t *h, int is_stdout)
{
char *name;
int i;
name = (char*)calloc(strlen(prefix) + 20, 1);
if (n >= 0) sprintf(name, "%s.%.4d.bam", prefix, n);
else sprintf(name, "%s.bam", prefix);
- assert(fp = bam_open(name, "w"));
+ fp = is_stdout? bam_dopen(fileno(stdout), "w") : bam_open(name, "w");
+ if (fp == 0) {
+ fprintf(stderr, "[sort_blocks] fail to create file %s.\n", name);
+ free(name);
+ // FIXME: possible memory leak
+ return;
+ }
free(name);
bam_header_write(fp, h);
for (i = 0; i < k; ++i)
and then merge them by calling bam_merge_core(). This function is
NOT thread safe.
*/
-void bam_sort_core(int is_by_qname, const char *fn, const char *prefix, size_t max_mem)
+void bam_sort_core_ext(int is_by_qname, const char *fn, const char *prefix, size_t max_mem, int is_stdout)
{
int n, ret, k, i;
size_t mem;
g_is_by_qname = is_by_qname;
n = k = 0; mem = 0;
fp = strcmp(fn, "-")? bam_open(fn, "r") : bam_dopen(fileno(stdin), "r");
- assert(fp);
+ if (fp == 0) {
+ fprintf(stderr, "[bam_sort_core] fail to open file %s\n", fn);
+ return;
+ }
header = bam_header_read(fp);
buf = (bam1_t**)calloc(max_mem / BAM_CORE_SIZE, sizeof(bam1_t*));
// write sub files
mem += ret;
++k;
if (mem >= max_mem) {
- sort_blocks(n++, k, buf, prefix, header);
+ sort_blocks(n++, k, buf, prefix, header, is_stdout);
mem = 0; k = 0;
}
}
if (ret != -1)
fprintf(stderr, "[bam_sort_core] truncated file. Continue anyway.\n");
- if (n == 0) sort_blocks(-1, k, buf, prefix, header);
+ if (n == 0) sort_blocks(-1, k, buf, prefix, header, is_stdout);
else { // then merge
char **fns, *fnout;
fprintf(stderr, "[bam_sort_core] merging from %d files...\n", n+1);
- sort_blocks(n++, k, buf, prefix, header);
+ sort_blocks(n++, k, buf, prefix, header, is_stdout);
fnout = (char*)calloc(strlen(prefix) + 20, 1);
- sprintf(fnout, "%s.bam", prefix);
+ if (is_stdout) sprintf(fnout, "-");
+ else sprintf(fnout, "%s.bam", prefix);
fns = (char**)calloc(n, sizeof(char*));
for (i = 0; i < n; ++i) {
fns[i] = (char*)calloc(strlen(prefix) + 20, 1);
sprintf(fns[i], "%s.%.4d.bam", prefix, i);
}
- bam_merge_core(is_by_qname, fnout, n, fns);
+ bam_merge_core(is_by_qname, fnout, 0, n, fns);
free(fnout);
for (i = 0; i < n; ++i) {
-// unlink(fns[i]);
+ unlink(fns[i]);
free(fns[i]);
}
free(fns);
bam_close(fp);
}
+void bam_sort_core(int is_by_qname, const char *fn, const char *prefix, size_t max_mem)
+{
+ bam_sort_core_ext(is_by_qname, fn, prefix, max_mem, 0);
+}
+
int bam_sort(int argc, char *argv[])
{
size_t max_mem = 500000000;
- int c, is_by_qname = 0;
- while ((c = getopt(argc, argv, "nm:")) >= 0) {
+ int c, is_by_qname = 0, is_stdout = 0;
+ while ((c = getopt(argc, argv, "nom:")) >= 0) {
switch (c) {
+ case 'o': is_stdout = 1; break;
case 'n': is_by_qname = 1; break;
case 'm': max_mem = atol(optarg); break;
}
}
if (optind + 2 > argc) {
- fprintf(stderr, "Usage: samtools sort [-n] [-m <maxMem>] <in.bam> <out.prefix>\n");
+ fprintf(stderr, "Usage: samtools sort [-on] [-m <maxMem>] <in.bam> <out.prefix>\n");
return 1;
}
- bam_sort_core(is_by_qname, argv[optind], argv[optind+1], max_mem);
+ bam_sort_core_ext(is_by_qname, argv[optind], argv[optind+1], max_mem, is_stdout);
return 0;
}
projects / samtools.git / blobdiff