#include <ctype.h>
#include "bam.h"
#include "khash.h"
+typedef char *str_p;
KHASH_MAP_INIT_STR(s, int)
+KHASH_MAP_INIT_STR(r2l, str_p)
void bam_aux_append(bam1_t *b, const char tag[2], char type, int len, uint8_t *data)
{
return bam_aux_get(b, tag);
}
-uint8_t *bam_aux_get(bam1_t *b, const char tag[2])
+#define __skip_tag(s) do { \
+ int type = toupper(*(s)); \
+ ++(s); \
+ if (type == 'C' || type == 'A') ++(s); \
+ else if (type == 'S') (s) += 2; \
+ else if (type == 'I' || type == 'F') (s) += 4; \
+ else if (type == 'D') (s) += 8; \
+ else if (type == 'Z' || type == 'H') { while (*(s)) ++(s); ++(s); } \
+ } while (0)
+
+uint8_t *bam_aux_get(const bam1_t *b, const char tag[2])
{
uint8_t *s;
int y = tag[0]<<8 | tag[1];
s = bam1_aux(b);
while (s < b->data + b->data_len) {
- int type, x = (int)s[0]<<8 | s[1];
+ int x = (int)s[0]<<8 | s[1];
s += 2;
if (x == y) return s;
- type = toupper(*s); ++s;
- if (type == 'C') ++s;
- else if (type == 'S') s += 2;
- else if (type == 'I' || type == 'F') s += 4;
- else if (type == 'D') s += 8;
- else if (type == 'Z' || type == 'H') { while (*s) putchar(*s++); ++s; }
+ __skip_tag(s);
}
return 0;
}
+// s MUST BE returned by bam_aux_get()
+int bam_aux_del(bam1_t *b, uint8_t *s)
+{
+ uint8_t *p, *aux;
+ aux = bam1_aux(b);
+ p = s - 2;
+ __skip_tag(s);
+ memmove(p, s, b->l_aux - (s - aux));
+ b->data_len -= s - p;
+ b->l_aux -= s - p;
+ return 0;
+}
void bam_init_header_hash(bam_header_t *header)
{
return k == kh_end(h)? -1 : kh_value(h, k);
}
-void bam_parse_region(bam_header_t *header, const char *str, int *ref_id, int *begin, int *end)
+int bam_parse_region(bam_header_t *header, const char *str, int *ref_id, int *begin, int *end)
{
char *s, *p;
int i, l, k;
iter = kh_get(s, h, s); /* get the ref_id */
if (iter == kh_end(h)) { // name not found
*ref_id = -1; free(s);
- return;
+ return -1;
}
*ref_id = kh_value(h, iter);
if (i == k) { /* dump the whole sequence */
*begin = 0; *end = 1<<29; free(s);
- return;
+ return -1;
}
for (p = s + i + 1; i != k; ++i) if (s[i] == '-') break;
*begin = atoi(p);
*end = atoi(p);
} else *end = 1<<29;
if (*begin > 0) --*begin;
- assert(*begin <= *end);
free(s);
+ if (*begin > *end) {
+ fprintf(stderr, "[bam_parse_region] invalid region.\n");
+ return -1;
+ }
+ return 0;
}
int32_t bam_aux2i(const uint8_t *s)
if (type == 'Z' || type == 'H') return (char*)s;
else return 0;
}
-
-char bam_aux_getCSi(bam1_t *b, int i)
-{
- uint8_t *c = bam_aux_get(b, "CS");
- char *cs = NULL;
-
- // return the base if the tag was not found
- if(0 == c) return 0;
-
- cs = bam_aux2Z(c);
- // adjust for strandedness and leading adaptor
- if(bam1_strand(b)) i = strlen(cs) - 1 - i;
- else i++;
- return cs[i];
-}
-
-char bam_aux_getCQi(bam1_t *b, int i)
-{
- uint8_t *c = bam_aux_get(b, "CQ");
- char *cq = NULL;
-
- // return the base if the tag was not found
- if(0 == c) return 0;
-
- cq = bam_aux2Z(c);
- // adjust for strandedness
- if(bam1_strand(b)) i = strlen(cq) - 1 - i;
- return cq[i];
-}
-
-char bam_aux_nt2int(char a)
-{
- switch(toupper(a)) {
- case 'A':
- return 0;
- break;
- case 'C':
- return 1;
- break;
- case 'G':
- return 2;
- break;
- case 'T':
- return 3;
- break;
- default:
- return 4;
- break;
- }
-}
-
-char bam_aux_ntnt2cs(char a, char b)
-{
- a = bam_aux_nt2int(a);
- b = bam_aux_nt2int(b);
- if(4 == a || 4 == b) return '4';
- return "0123"[(int)(a ^ b)];
-}
-
-char bam_aux_getCEi(bam1_t *b, int i)
-{
- int cs_i;
- uint8_t *c = bam_aux_get(b, "CS");
- char *cs = NULL;
- char prev_b, cur_b;
- char cur_color, cor_color;
-
- // return the base if the tag was not found
- if(0 == c) return 0;
-
- cs = bam_aux2Z(c);
-
- // adjust for strandedness and leading adaptor
- if(bam1_strand(b)) { //reverse strand
- cs_i = strlen(cs) - 1 - i;
- // get current color
- cur_color = cs[cs_i];
- // get previous base
- prev_b = (0 == cs_i) ? cs[0] : bam_nt16_rev_table[bam1_seqi(bam1_seq(b), i+1)];
- // get current base
- cur_b = bam_nt16_rev_table[bam1_seqi(bam1_seq(b), i)];
- }
- else {
- cs_i=i+1;
- // get current color
- cur_color = cs[cs_i];
- // get previous base
- prev_b = (0 == i) ? cs[0] : bam_nt16_rev_table[bam1_seqi(bam1_seq(b), i-1)];
- // get current base
- cur_b = bam_nt16_rev_table[bam1_seqi(bam1_seq(b), i)];
- }
-
- // corrected color
- cor_color = bam_aux_ntnt2cs(prev_b, cur_b);
-
- if(cur_color == cor_color) {
- return '-';
- }
- else {
- return cur_color;
- }
-}