* samtools-0.1.9-9 (r810)

[samtools.git] / bam2bcf_indel.c

diff --git a/bam2bcf_indel.c b/bam2bcf_indel.c
index b43a015c6e62adf0e9faf1341aec62a08b3c7040..ab9b499b1afeb4acd7f180002f679fddb8f9eddc 100644 (file)
--- a/bam2bcf_indel.c
+++ b/bam2bcf_indel.c
@@ -5,6 +5,7 @@
 #include "bam2bcf.h"
 #include "ksort.h"
 #include "kaln.h"
+#include "kprobaln.h"
 #include "khash.h"
 KHASH_SET_INIT_STR(rg)
 
@@ -235,8 +236,8 @@ int bcf_call_gap_prep(int n, int *n_plp, bam_pileup1_t **plp, int pos, bcf_calla
        bca->indelreg = 0;
        for (t = 0; t < n_types; ++t) {
                int l, ir;
-               ka_param2_t ap = ka_param2_qual;
-               ap.band_width = abs(types[t]) + 3;
+               kpa_par_t ap = { 1e-4, 1e-2, 10 };
+               ap.bw = abs(types[t]) + 3;
                // compute indelreg
                if (types[t] == 0) ir = 0;
                else if (types[t] > 0) ir = est_indelreg(pos, ref, types[t], &inscns[t*max_ins]);
@@ -263,6 +264,7 @@ int bcf_call_gap_prep(int n, int *n_plp, bam_pileup1_t **plp, int pos, bcf_calla
                                bam_pileup1_t *p = plp[s] + i;
                                int qbeg, qend, tbeg, tend, sc;
                                uint8_t *seq = bam1_seq(p->b);
+                               // FIXME: the following skips soft clips, but using them may be more sensitive.
                                // determine the start and end of sequences for alignment
                                qbeg = tpos2qpos(&p->b->core, bam1_cigar(p->b), left,  0, &tbeg);
                                qend = tpos2qpos(&p->b->core, bam1_cigar(p->b), right, 1, &tend);
@@ -273,10 +275,19 @@ int bcf_call_gap_prep(int n, int *n_plp, bam_pileup1_t **plp, int pos, bcf_calla
                                // write the query sequence
                                for (l = qbeg; l < qend; ++l)
                                        query[l - qbeg] = bam_nt16_nt4_table[bam1_seqi(seq, l)];
-                               // do alignment; this takes most of computing time for indel calling
-                               sc = ka_global_score((uint8_t*)ref2 + tbeg - left, tend - tbeg + abs(types[t]),
-                                                                        (uint8_t*)query, qend - qbeg, &ap);
-                               score[K*n_types + t] = -sc;
+                               { // do alignment; this is the bottleneck
+                                       const uint8_t *qual = bam1_qual(p->b), *bq;
+                                       uint8_t *qq = 0;
+                                       qq = calloc(qend - qbeg, 1);
+                                       bq = (uint8_t*)bam_aux_get(p->b, "BQ");
+                                       if (bq) ++bq;
+                                       for (l = qbeg; l < qend; ++l)
+                                               qq[l - qbeg] = bq? qual[l] + (bq[l] - 33) : qual[l];
+                                       sc = kpa_glocal((uint8_t*)ref2 + tbeg - left, tend - tbeg + abs(types[t]),
+                                                                       (uint8_t*)query, qend - qbeg, qq, &ap, 0, 0);
+                                       score[K*n_types + t] = sc;
+                                       free(qq);
+                               }
 /*
                                for (l = 0; l < tend - tbeg + abs(types[t]); ++l)
                                        fputc("ACGTN"[(int)ref2[tbeg-left+l]], stderr);