#include <string.h>
+#include <unistd.h>
+#include "faidx.h"
#include "sam.h"
#define TYPE_BAM 1
int i;
h = bam_header_init();
*h = *h0;
- h->hash = 0;
+ h->hash = h->dict = h->rg2lib = 0;
h->text = (char*)calloc(h->l_text + 1, 1);
memcpy(h->text, h0->text, h->l_text);
h->target_len = (uint32_t*)calloc(h->n_targets, 4);
}
return h;
}
+static void append_header_text(bam_header_t *header, char* text, int len)
+{
+ int x = header->l_text + 1;
+ int y = header->l_text + len + 1; // 1 byte null
+ if (text == 0) return;
+ kroundup32(x);
+ kroundup32(y);
+ if (x < y) header->text = (char*)realloc(header->text, y);
+ strncpy(header->text + header->l_text, text, len); // we cannot use strcpy() here.
+ header->l_text += len;
+ header->text[header->l_text] = 0;
+}
+
+int samthreads(samfile_t *fp, int n_threads, int n_sub_blks)
+{
+ if (!(fp->type&1) || (fp->type&2)) return -1;
+ bgzf_mt(fp->x.bam, n_threads, n_sub_blks);
+ return 0;
+}
samfile_t *samopen(const char *fn, const char *mode, const void *aux)
{
samfile_t *fp;
fp = (samfile_t*)calloc(1, sizeof(samfile_t));
- if (mode[0] == 'r') { // read
+ if (strchr(mode, 'r')) { // read
fp->type |= TYPE_READ;
- if (mode[1] == 'b') { // binary
+ if (strchr(mode, 'b')) { // binary
fp->type |= TYPE_BAM;
fp->x.bam = strcmp(fn, "-")? bam_open(fn, "r") : bam_dopen(fileno(stdin), "r");
if (fp->x.bam == 0) goto open_err_ret;
fp->header = sam_header_read(fp->x.tamr);
if (fp->header->n_targets == 0) { // no @SQ fields
if (aux) { // check if aux is present
- bam_header_destroy(fp->header);
+ bam_header_t *textheader = fp->header;
fp->header = sam_header_read2((const char*)aux);
+ if (fp->header == 0) goto open_err_ret;
+ append_header_text(fp->header, textheader->text, textheader->l_text);
+ bam_header_destroy(textheader);
}
- if (fp->header->n_targets == 0)
- fprintf(stderr, "[samopen] empty header.\n");
- } else fprintf(stderr, "[samopen] SAM header is present: %d sequences.\n", fp->header->n_targets);
+ if (fp->header->n_targets == 0 && bam_verbose >= 1)
+ fprintf(stderr, "[samopen] no @SQ lines in the header.\n");
+ } else if (bam_verbose >= 2) fprintf(stderr, "[samopen] SAM header is present: %d sequences.\n", fp->header->n_targets);
}
- } else if (mode[0] == 'w') { // write
+ } else if (strchr(mode, 'w')) { // write
fp->header = bam_header_dup((const bam_header_t*)aux);
- if (mode[1] == 'b') { // binary
+ if (strchr(mode, 'b')) { // binary
+ char bmode[3];
+ int i, compress_level = -1;
+ for (i = 0; mode[i]; ++i) if (mode[i] >= '0' && mode[i] <= '9') break;
+ if (mode[i]) compress_level = mode[i] - '0';
+ if (strchr(mode, 'u')) compress_level = 0;
+ bmode[0] = 'w'; bmode[1] = compress_level < 0? 0 : compress_level + '0'; bmode[2] = 0;
fp->type |= TYPE_BAM;
- fp->x.bam = strcmp(fn, "-")? bam_open(fn, "w") : bam_dopen(fileno(stdout), "w");
+ fp->x.bam = strcmp(fn, "-")? bam_open(fn, bmode) : bam_dopen(fileno(stdout), bmode);
if (fp->x.bam == 0) goto open_err_ret;
bam_header_write(fp->x.bam, fp->header);
} else { // text
// open file
fp->x.tamw = strcmp(fn, "-")? fopen(fn, "w") : stdout;
- if (fp->x.tamr == 0) goto open_err_ret;
+ if (fp->x.tamw == 0) goto open_err_ret;
+ if (strchr(mode, 'X')) fp->type |= BAM_OFSTR<<2;
+ else if (strchr(mode, 'x')) fp->type |= BAM_OFHEX<<2;
+ else fp->type |= BAM_OFDEC<<2;
// write header
- if (strstr(mode, "h")) {
+ if (strchr(mode, 'h')) {
int i;
bam_header_t *alt;
// parse the header text
sam_header_parse(alt);
alt->l_text = 0; alt->text = 0;
// check if there are @SQ lines in the header
+ fwrite(fp->header->text, 1, fp->header->l_text, fp->x.tamw); // FIXME: better to skip the trailing NULL
if (alt->n_targets) { // then write the header text without dumping ->target_{name,len}
- if (alt->n_targets != fp->header->n_targets)
- fprintf(stderr, "[samopen] inconsistent number of target sequences.\n");
- fwrite(fp->header->text, 1, fp->header->l_text, fp->x.tamw);
+ if (alt->n_targets != fp->header->n_targets && bam_verbose >= 1)
+ fprintf(stderr, "[samopen] inconsistent number of target sequences. Output the text header.\n");
} else { // then dump ->target_{name,len}
for (i = 0; i < fp->header->n_targets; ++i)
fprintf(fp->x.tamw, "@SQ\tSN:%s\tLN:%d\n", fp->header->target_name[i], fp->header->target_len[i]);
if (fp == 0 || (fp->type & TYPE_READ)) return -1; // not open for writing
if (fp->type & TYPE_BAM) return bam_write1(fp->x.bam, b);
else {
- char *s = bam_format1(fp->header, b);
+ char *s = bam_format1_core(fp->header, b, fp->type>>2&3);
int l = strlen(s);
fputs(s, fp->x.tamw); fputc('\n', fp->x.tamw);
free(s);
bam_destroy1(b);
return 0;
}
+
+char *samfaipath(const char *fn_ref)
+{
+ char *fn_list = 0;
+ if (fn_ref == 0) return 0;
+ fn_list = calloc(strlen(fn_ref) + 5, 1);
+ strcat(strcpy(fn_list, fn_ref), ".fai");
+ if (access(fn_list, R_OK) == -1) { // fn_list is unreadable
+ if (access(fn_ref, R_OK) == -1) {
+ fprintf(stderr, "[samfaipath] fail to read file %s.\n", fn_ref);
+ } else {
+ if (bam_verbose >= 3) fprintf(stderr, "[samfaipath] build FASTA index...\n");
+ if (fai_build(fn_ref) == -1) {
+ fprintf(stderr, "[samfaipath] fail to build FASTA index.\n");
+ free(fn_list); fn_list = 0;
+ }
+ }
+ }
+ return fn_list;
+}