#!/usr/bin/env ruby # Copyright (C) 2007-2011 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Trim sequence ends for residues with a low quality score. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'pp' casts = [] casts << {:long=>'min', :short=>'m', :type=>'uint', :mandatory=>true, :default=>15, :allowed=>nil, :disallowed=>'0'} casts << {:long=>'trim', :short=>'t', :type=>'string', :mandatory=>true, :default=>'both', :allowed=>'left,right,both', :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) ILLUMINA_BASE = 64 regex_left = Regexp.new("^[#{(ILLUMINA_BASE).chr}-#{(ILLUMINA_BASE + options[:min]).chr}]+") regex_right = Regexp.new("[#{(ILLUMINA_BASE).chr}-#{(ILLUMINA_BASE + options[:min]).chr}]+$") Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| input.each_record do |record| if record.has_key? :SEQ and record.has_key? :SCORES case options[:trim] when /both/ record[:SCORES].match(regex_right) do |m| record[:SEQ] = record[:SEQ][0 ... record[:SEQ].length - m.to_s.length] record[:SCORES] = $` record[:SEQ_LEN] = record[:SEQ].length record[:TRIM_LEFT] = m.to_s.length end record[:SCORES].match(regex_left) do |m| record[:SEQ] = record[:SEQ][m.to_s.length ... record[:SEQ].length] record[:SCORES] = $' record[:SEQ_LEN] = record[:SEQ].length record[:TRIM_RIGHT] = $'.length - 1 end when /left/ record[:SCORES].match(regex_left) do |m| record[:SEQ] = record[:SEQ][m.to_s.length ... record[:SEQ].length] record[:SCORES] = $' record[:SEQ_LEN] = record[:SEQ].length record[:TRIM_LEFT] = m.to_s.length end when /right/ record[:SCORES].match(regex_right) do |m| record[:SEQ] = record[:SEQ][0 ... record[:SEQ].length - m.to_s.length] record[:SCORES] = $` record[:SEQ_LEN] = record[:SEQ].length record[:TRIM_RIGHT] = $`.length - 1 end end end output.puts record end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__