#!/usr/bin/env ruby # Copyright (C) 2007-2013 Martin A. Hansen. # This program is free software; you can redistribute it and/or # modify it under the terms of the GNU General Public License # as published by the Free Software Foundation; either version 2 # of the License, or (at your option) any later version. # This program is distributed in the hope that it will be useful, # but WITHOUT ANY WARRANTY; without even the implied warranty of # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the # GNU General Public License for more details. # You should have received a copy of the GNU General Public License # along with this program; if not, write to the Free Software # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. # http://www.gnu.org/copyleft/gpl.html # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # This program is part of the Biopieces framework (www.biopieces.org). # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # Read FASTQ entries from one or more files. # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< require 'maasha/biopieces' require 'maasha/fastq' allowed_enc = 'auto,sanger,solexa,illumina1.3,illumina1.5,illumina1.8' casts = [] casts << {:long=>'data_in', :short=>'i', :type=>'files!', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>nil} casts << {:long=>'num', :short=>'n', :type=>'uint', :mandatory=>false, :default=>nil, :allowed=>nil, :disallowed=>'0'} casts << {:long=>'encoding', :short=>'e', :type=>'string', :mandatory=>false, :default=>'auto', :allowed=>allowed_enc, :disallowed=>nil} options = Biopieces.options_parse(ARGV, casts) num = 0 first = true last = false Biopieces.open(options[:stream_in], options[:stream_out]) do |input, output| unless options[:data_in] and options[:data_in].first == '-' input.each_record do |record| output.puts record end end if options[:data_in] options[:data_in].each do |file| encoding = options[:encoding].downcase.delete('.') Fastq.open(file, mode='r') do |fastq| fastq.each do |entry| if encoding == 'auto' if entry.qual_base33? # sanger or illumina18 encoding = 'illumina18' elsif entry.qual_base64? # solexa or illumina13 or illumina15 encoding = 'illumina13' else raise SeqError, "Could not auto-detect quality score encoding" end end entry.convert_scores!(encoding, 'illumina13') if first # raise SeqError, "Quality score outside valid range" unless entry.qual_valid?('illumina13') first = false end output.puts entry.to_bp num += 1 if options[:num] == num last = true break end end end break if last end end end # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< __END__