1 // ***************************************************************************
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2 // BamMultiReader.h (c) 2010 Erik Garrison
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3 // Marth Lab, Department of Biology, Boston College
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4 // All rights reserved.
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5 // ---------------------------------------------------------------------------
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6 // Last modified: 3 September 2010 (DB)
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7 // ---------------------------------------------------------------------------
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8 // Functionality for simultaneously reading multiple BAM files
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9 // ***************************************************************************
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11 #ifndef BAMMULTIREADER_H
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12 #define BAMMULTIREADER_H
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17 #include <utility> // for pair
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20 using namespace std;
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22 // BamTools includes
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24 #include "BamReader.h"
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26 namespace BamTools {
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28 // index mapping reference/position pairings to bamreaders and their alignments
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29 typedef multimap<pair<int, int>, pair<BamReader*, BamAlignment*> > AlignmentIndex;
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32 class BamMultiReader {
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34 // constructor / destructor
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36 BamMultiReader(void);
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37 ~BamMultiReader(void);
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46 // region under analysis, specified using SetRegion
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49 // ----------------------
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50 // BAM file operations
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51 // ----------------------
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56 // opens BAM files (and optional BAM index files, if provided)
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57 // @openIndexes - triggers index opening, useful for suppressing
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58 // error messages during merging of files in which we may not have
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60 // @coreMode - setup our first alignments using GetNextAlignmentCore();
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61 // also useful for merging
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62 // @preferStandardIndex - look for standard BAM index ".bai" first. If false,
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63 // will look for BamTools index ".bti".
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64 bool Open(const vector<string> filenames, bool openIndexes = true, bool coreMode = false, bool preferStandardIndex = false);
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66 // returns whether underlying BAM readers ALL have an index loaded
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67 // this is useful to indicate whether Jump() or SetRegion() are possible
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68 bool IsIndexLoaded(void) const;
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70 // performs random-access jump to reference, position
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71 bool Jump(int refID, int position = 0);
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73 // sets the target region
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74 bool SetRegion(const BamRegion& region);
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75 bool SetRegion(const int&, const int&, const int&, const int&); // convenience function to above
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77 // returns file pointers to beginning of alignments
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80 // ----------------------
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81 // access alignment data
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82 // ----------------------
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83 // updates the reference id marker to match the lower limit of our readers
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84 void UpdateReferenceID(void);
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86 // retrieves next available alignment (returns success/fail) from all files
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87 bool GetNextAlignment(BamAlignment&);
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88 // retrieves next available alignment (returns success/fail) from all files
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89 // and populates the support data with information about the alignment
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90 // *** BUT DOES NOT PARSE CHARACTER DATA FROM THE ALIGNMENT
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91 bool GetNextAlignmentCore(BamAlignment&);
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92 // ... should this be private?
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93 bool HasOpenReaders(void);
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95 // ----------------------
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96 // access auxiliary data
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97 // ----------------------
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99 // returns unified SAM header text for all files
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100 const string GetHeaderText(void) const;
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101 // returns number of reference sequences
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102 const int GetReferenceCount(void) const;
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103 // returns vector of reference objects
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104 const BamTools::RefVector GetReferenceData(void) const;
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105 // returns reference id (used for BamMultiReader::Jump()) for the given reference name
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106 const int GetReferenceID(const std::string& refName) const;
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107 // validates that we have a congruent set of BAM files that are aligned against the same reference sequences
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108 void ValidateReaders() const;
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110 // ----------------------
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111 // BAM index operations
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112 // ----------------------
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114 // creates index for BAM files which lack them, saves to files (default = bamFilename + ".bai")
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115 bool CreateIndexes(bool useDefaultIndex = true);
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117 //const int GetReferenceID(const string& refName) const;
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120 void PrintFilenames(void);
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121 void DumpAlignmentIndex(void);
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122 void UpdateAlignments(void); // updates our alignment cache
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124 // private implementation
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127 // the set of readers and alignments which we operate on, maintained throughout the life of this class
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128 vector<pair<BamReader*, BamAlignment*> > readers;
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130 // readers and alignments sorted by reference id and position, to keep track of the lowest (next) alignment
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131 // when a reader reaches EOF, its entry is removed from this index
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132 AlignmentIndex alignments;
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134 vector<string> fileNames;
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137 } // namespace BamTools
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139 #endif // BAMMULTIREADER_H
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