3 # Copyright (C) 2007-2009 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Write tabular BLAST output from the stream.
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
32 use Maasha::Biopieces;
35 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
38 my ( $options, $in, $out, $record, $first, $entry, $data_out );
40 $options = Maasha::Biopieces::parse_options(
42 { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
43 { long => 'data_out', short => 'o', type => 'file', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
44 { long => 'comment', short => 'c', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
45 { long => 'compress', short => 'Z', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
49 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
50 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
51 $data_out = Maasha::Biopieces::write_stream( $options->{ "data_out" }, $options->{ "compress" } ) ;
55 while ( $record = Maasha::Biopieces::get_record( $in ) )
57 if ( $entry = biopiece2blast_tab( $record ) )
59 if ( $options->{ "comment" } and $first )
61 print $data_out "# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score\n";
66 print $data_out join( "\t", @{ $entry } ), "\n";
69 Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" };
74 Maasha::Biopieces::close_stream( $in );
75 Maasha::Biopieces::close_stream( $out );
78 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> SUBROUTINES <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
81 sub biopiece2blast_tab
83 # Martin A. Hansen, May 2009.
85 # Convert a Biopiece record to a BLAST tabular entry.
86 # Note that we correct positions because BLAST is 1-based.
88 my ( $record, # hashref
93 my ( @fields, $s_beg, $s_end );
95 return undef if not defined $record->{ 'REC_TYPE' } or not $record->{ 'REC_TYPE' } eq "BLAST";
97 if ( $record->{ "STRAND" } eq '+' )
99 $s_beg = $record->{ "S_BEG" };
100 $s_end = $record->{ "S_END" };
104 $s_beg = $record->{ "S_END" };
105 $s_end = $record->{ "S_BEG" };
108 $fields[ 0 ] = $record->{ "Q_ID" };
109 $fields[ 1 ] = $record->{ "S_ID" };
110 $fields[ 2 ] = $record->{ "IDENT" };
111 $fields[ 3 ] = $record->{ "ALIGN_LEN" };
112 $fields[ 4 ] = $record->{ "MISMATCHES" };
113 $fields[ 5 ] = $record->{ "GAPS" };
114 $fields[ 6 ] = $record->{ "Q_BEG" } + 1;
115 $fields[ 7 ] = $record->{ "Q_END" } + 1;
116 $fields[ 8 ] = $s_beg + 1;
117 $fields[ 9 ] = $s_end + 1;
118 $fields[ 10 ] = $record->{ "E_VAL" };
119 $fields[ 11 ] = $record->{ "BIT_SCORE" };
121 return wantarray ? @fields : \@fields;
125 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
130 Maasha::Biopieces::status_set();
136 Maasha::Biopieces::status_log();
140 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<