3 # Copyright (C) 2007-2010 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Write sequences and scores from stream to FASTA and QUAL files.
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
31 use Maasha::Biopieces;
36 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
39 my ( $options, $in, $out, $record, $data_out, $qual_out, $entry );
41 $options = Maasha::Biopieces::parse_options(
43 { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
44 { long => 'data_out', short => 'o', type => 'file', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
45 { long => 'qual_out', short => 'q', type => 'file', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
46 { long => 'compress', short => 'Z', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
50 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
51 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
53 $data_out = Maasha::Biopieces::write_stream( $options->{ "data_out" }, $options->{ "compress" } );
54 $qual_out = Maasha::Biopieces::write_stream( $options->{ "qual_out" }, $options->{ "compress" } );
56 while ( $record = Maasha::Biopieces::get_record( $in ) )
58 if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) )
60 Maasha::Fasta::put_entry( $entry, $data_out );
62 qual_put_entry( $entry->[ 0 ], $record->{ 'SCORES' }, $qual_out );
65 Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" };
71 Maasha::Biopieces::close_stream( $in );
72 Maasha::Biopieces::close_stream( $out );
75 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
80 # Martin A. Hansen, May 2010.
82 # Output a score string in 454 quality format.
84 my ( $name, # entry name
85 $score_str, # scores in Solexa format.
86 $fh, # output filehandle
91 $score_str = Maasha::Fastq::solexa_str2dec_str( $score_str );
92 $score_str =~ tr/;/ /;
95 print $fh "$score_str\n";
99 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
104 Maasha::Biopieces::status_set();
110 Maasha::Biopieces::status_log();
114 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<