3 # Copyright (C) 2007-2010 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Trim sequence ends for residues with a low quality score.
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
31 use Maasha::Biopieces;
35 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
38 my ( $options, $in, $out, $record, $left, $right, $pos_l, $pos_r );
40 $options = Maasha::Biopieces::parse_options(
42 { long => 'min', short => 'm', type => 'uint', mandatory => 'no', default => 15, allowed => undef, disallowed => 0 },
43 { long => 'trim', short => 't', type => 'string', mandatory => 'no', default => 'both', allowed => 'left,right,both', disallowed => undef },
47 $right = 1 if $options->{ 'trim' } eq 'right';
48 $left = 1 if $options->{ 'trim' } eq 'left';
49 $right = 1 if $options->{ 'trim' } eq 'both';
50 $left = 1 if $options->{ 'trim' } eq 'both';
52 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
53 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
55 while ( $record = Maasha::Biopieces::get_record( $in ) )
57 if ( exists $record->{ 'SEQ' } and exists $record->{ 'SCORES' } )
61 $pos_r = Maasha::Fastq::trim_right( $record->{ 'SCORES' }, $options->{ 'min' } );
63 $record->{ 'SEQ' } = substr $record->{ 'SEQ' }, 0, $pos_r + 1;
64 $record->{ 'SCORES' } = substr $record->{ 'SCORES' }, 0, $pos_r + 1;
65 $record->{ 'TRIM_RIGHT' } = $pos_r;
70 $pos_l = Maasha::Fastq::trim_left( $record->{ 'SCORES' }, $options->{ 'min' } );
72 $record->{ 'SEQ' } = substr $record->{ 'SEQ' }, $pos_l;
73 $record->{ 'SCORES' } = substr $record->{ 'SCORES' }, $pos_l;
74 $record->{ 'TRIM_LEFT' } = $pos_l;
77 $record->{ 'SEQ_LEN' } = length $record->{ 'SEQ' };
80 Maasha::Biopieces::put_record( $record, $out );
83 Maasha::Biopieces::close_stream( $in );
84 Maasha::Biopieces::close_stream( $out );
87 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
92 Maasha::Biopieces::status_set();
98 Maasha::Biopieces::status_log();
102 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<