3 # Copyright (C) 2007-2009 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Format a genome creating specified indexes.
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
32 use Maasha::Biopieces;
39 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
42 my ( $default, $formats, $options, $in, $out, $record, $data_out, $entry,
43 $genome, $dir, $fasta_dir, $phastcons_dir, $fh_out, $vals, $format, $tmp_dir );
45 $tmp_dir = Maasha::Biopieces::get_tmpdir();
46 $default = $ENV{ 'BP_DATA' };
47 $formats = 'fasta,blast,vmatch,bowtie,phastcons';
49 $options = Maasha::Biopieces::parse_options(
51 { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
52 { long => 'dir', short => 'd', type => 'dir!', mandatory => 'no', default => $default, allowed => undef, disallowed => undef },
53 { long => 'genome', short => 'g', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
54 { long => 'formats', short => 'f', type => 'list', mandatory => 'yes', default => undef, allowed => $formats, disallowed => '0' },
58 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
59 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
61 $dir = $options->{ 'dir' };
62 $genome = $options->{ 'genome' };
64 Maasha::Filesys::dir_create_if_not_exists( "$dir/genomes" );
65 Maasha::Filesys::dir_create_if_not_exists( "$dir/genomes/$genome" );
67 if ( grep { $_ =~ /fasta|blast|vmatch|bowtie/i } @{ $options->{ "formats" } } )
69 if ( -f "$dir/genomes/$genome/fasta/$genome.fna" )
71 $fasta_dir = "$dir/genomes/$genome/fasta";
75 Maasha::Filesys::dir_create_if_not_exists( "$dir/genomes/$genome/fasta" );
77 $fasta_dir = "$dir/genomes/$genome/fasta";
79 $fh_out = Maasha::Filesys::file_write_open( "$fasta_dir/$genome.fna" );
82 elsif ( grep { $_ =~ /phastcons/i } @{ $options->{ "formats" } } )
84 Maasha::Filesys::dir_create_if_not_exists( "$dir/genomes/$genome/phastcons" );
86 $phastcons_dir = "$dir/genomes/$genome/phastcons";
88 $fh_out = Maasha::Filesys::file_write_open( "$phastcons_dir/$genome.pp" );
91 while ( $record = Maasha::Biopieces::get_record( $in ) )
93 if ( $fh_out and $entry = Maasha::Fasta::biopiece2fasta( $record ) )
95 Maasha::Fasta::put_entry( $entry, $fh_out );
97 elsif ( $fh_out and $record->{ "CHR" } and $record->{ "CHR_BEG" } and $record->{ "STEP" } and $record->{ "VALS" } )
99 print $fh_out "fixedStep chrom=$record->{ 'CHR' } start=$record->{ 'CHR_BEG' } step=$record->{ 'STEP' }\n";
101 $vals = $record->{ 'VALS' };
105 print $fh_out "$vals\n";
108 Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" };
111 foreach $format ( @{ $options->{ 'formats' } } )
113 print STDERR qq(Creating format: $format for $genome ... ) if $options->{ 'verbose' };
115 if ( $format =~ /^fasta$/i ) { Maasha::Fasta::fasta_index( "$fasta_dir/$genome.fna", "$dir/genomes/$genome/fasta/$genome.index" ) }
116 elsif ( $format =~ /^blast$/i ) { Maasha::NCBI::blast_index( "$genome.fna", $fasta_dir, "$dir/genomes/$genome/blast", "dna", $genome ) }
117 elsif ( $format =~ /^blat$/i ) { warn "BLAT FORMAT NOT IMPLEMENTED" }
118 elsif ( $format =~ /^vmatch$/i ) { Maasha::Match::vmatch_index( "$genome.fna", $fasta_dir, "$dir/genomes/$genome/vmatch", $tmp_dir ) }
119 elsif ( $format =~ /^bowtie$/i ) { Maasha::Bowtie::bowtie_index( "$fasta_dir/$genome.fna", "$dir/genomes/$genome/bowtie", $genome, $options->{ 'verbose' } ) }
120 elsif ( $format =~ /^phastcons$/i ) { Maasha::UCSC::phastcons_index( "$genome.pp", $phastcons_dir ) }
122 print STDERR qq(done.\n) if $options->{ 'verbose' };
125 close $fh_out if $fh_out;
127 Maasha::Biopieces::close_stream( $in );
128 Maasha::Biopieces::close_stream( $out );
131 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
136 Maasha::Biopieces::status_set();
142 Maasha::Biopieces::status_log();
146 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<