3 # Copyright (C) 2007-2009 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Create a BLAST database from sequences in stream for use with [blast_seq].
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
33 use Maasha::Biopieces;
36 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
39 my ( $options, $in, $out, $record, $tmp_dir, $file_tmp, $fh_tmp, $entry, $seq_type, $arg );
41 $options = Maasha::Biopieces::parse_options(
43 { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
44 { long => 'directory', short => 'd', type => 'dir', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
45 { long => 'index_name', short => 'i', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
49 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
50 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
52 $tmp_dir = Maasha::Biopieces::get_tmpdir();
53 $file_tmp = "$tmp_dir/$options->{ 'index_name' }";
54 $fh_tmp = Maasha::Filesys::file_write_open( $file_tmp );
56 while ( $record = Maasha::Biopieces::get_record( $in ) )
58 if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) )
60 $seq_type = Maasha::Seq::seq_guess_type( $record->{ 'SEQ' } ) if not $seq_type;
62 Maasha::Fasta::put_entry( $entry, $fh_tmp );
65 Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" };
70 $arg = "-t $options->{ 'index_name' }";
72 if ( $seq_type eq "PROTEIN" ) {
78 $arg .= " -i $file_tmp";
80 if ( $options->{ 'verbose' } )
82 print STDERR "Running: formatdb $arg\n";
84 Maasha::Common::run( "formatdb", "$arg" );
88 Maasha::Common::run( "formatdb", "$arg -l /dev/null" );
91 Maasha::Filesys::dir_create_if_not_exists( $options->{ 'directory' } );
95 system( "cp $tmp_dir/* $options->{ 'directory' }" );
97 Maasha::Biopieces::close_stream( $in );
98 Maasha::Biopieces::close_stream( $out );
101 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
106 Maasha::Biopieces::status_set();
112 Maasha::Biopieces::status_log();
116 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<