3 # Copyright (C) 2007-2009 Martin A. Hansen.
5 # This program is free software; you can redistribute it and/or
6 # modify it under the terms of the GNU General Public License
7 # as published by the Free Software Foundation; either version 2
8 # of the License, or (at your option) any later version.
10 # This program is distributed in the hope that it will be useful,
11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
13 # GNU General Public License for more details.
15 # You should have received a copy of the GNU General Public License
16 # along with this program; if not, write to the Free Software
17 # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA.
19 # http://www.gnu.org/copyleft/gpl.html
22 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DESCRIPTION <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
24 # Write Biopiece records to the Biopieces Genome browser.
26 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
34 use Maasha::Biopieces;
36 use Maasha::BGB::Track;
37 use Maasha::BGB::Wiggle;
48 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
51 my ( $data_dir, $user, $options, $path, $in, $out, $tmp_dir, %fh_hash, $fh_out, $record, $entry, $key, $dst_dir, @nums, $num, $contig_dir, $wig );
53 $data_dir = Maasha::Biopieces::biopiecesrc( "BGB_DATA_DIR" );
54 $user = Maasha::Biopieces::biopiecesrc( "BGB_USER" );
56 $options = Maasha::Biopieces::parse_options(
58 { long => 'user', short => 'u', type => 'string', mandatory => 'no', default => $user, allowed => undef, disallowed => undef },
59 { long => 'clade', short => 'c', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
60 { long => 'genome', short => 'g', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
61 { long => 'assembly', short => 'a', type => 'string', mandatory => 'yes', default => undef, allowed => undef, disallowed => undef },
62 { long => 'track_name', short => 't', type => 'string', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
63 { long => 'track_type', short => 'T', type => 'string', mandatory => 'no', default => 'linear', allowed => 'linear,wiggle', disallowed => undef },
64 { long => 'force', short => 'f', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
65 { long => 'no_stream', short => 'x', type => 'flag', mandatory => 'no', default => undef, allowed => undef, disallowed => undef },
69 $in = Maasha::Biopieces::read_stream( $options->{ "stream_in" } );
70 $out = Maasha::Biopieces::write_stream( $options->{ "stream_out" } );
72 if ( $options->{ 'track_name' } )
74 $options->{ 'track_name' } =~ tr/ /_/;
76 $path = join "/", $data_dir, "Users", $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' };
78 Maasha::Common::error( qq(Path not found: "$path") ) if not -d $path;
80 $tmp_dir = Maasha::Biopieces::get_tmpdir();
82 while ( $record = Maasha::Biopieces::get_record( $in ) )
84 if ( $entry = Maasha::KISS::biopiece2kiss( $record ) )
86 $entry->[ S_ID ] = ( split / /, $entry->[ S_ID ] )[ 0 ];
88 if ( not exists $fh_hash{ $entry->[ S_ID ] } ) {
89 $fh_hash{ $entry->[ S_ID ] } = Maasha::Filesys::file_write_open( "$tmp_dir/$entry->[ S_ID ]" );
92 $fh_out = $fh_hash{ $entry->[ S_ID ] };
94 Maasha::KISS::kiss_entry_put( $entry, $fh_out );
97 Maasha::Biopieces::put_record( $record, $out ) if not $options->{ "no_stream" };
100 $num = Maasha::BGB::Track::max_track( $options->{ 'user' }, $options->{ 'clade' }, $options->{ 'genome' }, $options->{ 'assembly' } );
101 $num = sprintf( "%04d", $num + 10 );
103 foreach $key ( keys %fh_hash )
105 close $fh_hash{ $key };
107 $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$path/$key" );
108 $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$dst_dir/Tracks" );
110 $dst_dir = "$dst_dir/$num" . "_$options->{ 'track_name' }";
112 Maasha::Filesys::dir_create( $dst_dir );
114 if ( $options->{ 'track_type' } eq 'linear' )
116 Maasha::Filesys::file_copy( "$tmp_dir/$key", "$dst_dir/track_data.kiss" );
118 Maasha::KISS::kiss_sort( "$dst_dir/track_data.kiss" );
120 elsif ( $options->{ 'track_type' } eq 'wiggle' )
122 $wig = Maasha::BGB::Wiggle::wiggle_encode( "$tmp_dir/$key" );
123 Maasha::BGB::Wiggle::wiggle_store( "$dst_dir/track_data.wig", $wig );
126 unlink "$tmp_dir/$key";
131 $path = join "/", $data_dir, "Users/", $options->{ 'user' }, $options->{ 'clade' };
133 Maasha::Common::error( qq(Path not found: "$path") ) if not -d $path;
135 $dst_dir = Maasha::Filesys::dir_create_if_not_exists( "$path/$options->{ 'genome' }" );
136 $dst_dir = Maasha::Filesys::dir_create( "$dst_dir/$options->{ 'assembly' }" );
138 while ( $record = Maasha::Biopieces::get_record( $in ) )
140 if ( $entry = Maasha::Fasta::biopiece2fasta( $record ) )
142 $entry->[ SEQ_NAME ] = ( split " ", $entry->[ SEQ_NAME ] )[ 0 ];
144 if ( $options->{ 'force' } ) {
145 Maasha::Filesys::dir_remove( "$dst_dir/$entry->[ SEQ_NAME ]" );
148 $contig_dir = Maasha::Filesys::dir_create( "$dst_dir/$entry->[ SEQ_NAME ]" );
149 $contig_dir = Maasha::Filesys::dir_create( "$contig_dir/Sequence" );
151 $fh_out = Maasha::Filesys::file_write_open( "$contig_dir/sequence.txt" );
152 print $fh_out $entry->[ SEQ ];
158 Maasha::Biopieces::close_stream( $in );
159 Maasha::Biopieces::close_stream( $out );
162 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<
167 Maasha::Biopieces::status_set();
173 Maasha::Biopieces::status_log();
177 # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<