6 #ifndef NCURSES_VERSION
7 #warning "_CURSES_LIB=1 but NCURSES_VERSION not defined; tview is NOT compiled"
11 #elif _CURSES_LIB == 2
15 #warning "_CURSES_LIB is not 0, 1 or 2; tview is NOT compiled"
24 #include "bam_maqcns.h"
26 char bam_aux_getCEi(bam1_t *b, int i);
27 char bam_aux_getCSi(bam1_t *b, int i);
28 char bam_aux_getCQi(bam1_t *b, int i);
30 #define TV_MIN_ALNROW 2
31 #define TV_MAX_GOTO 40
32 #define TV_LOW_MAPQ 10
34 #define TV_COLOR_MAPQ 0
35 #define TV_COLOR_BASEQ 1
36 #define TV_COLOR_NUCL 2
37 #define TV_COLOR_COL 3
38 #define TV_COLOR_COLQ 4
40 #define TV_BASE_NUCL 0
41 #define TV_BASE_COLOR_SPACE 1
45 WINDOW *wgoto, *whelp;
51 int curr_tid, left_pos;
55 int ccol, last_pos, row_shift, base_for, color_for, is_dot, l_ref, ins, no_skip;
59 int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void *data)
61 tview_t *tv = (tview_t*)data;
62 int i, j, c, rb, attr, max_ins = 0;
64 if (pos < tv->left_pos || tv->ccol > tv->mcol) return 0; // out of screen
66 rb = (tv->ref && pos - tv->left_pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N';
67 for (i = tv->last_pos + 1; i < pos; ++i) {
68 if (i%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", i+1);
69 c = tv->ref? tv->ref[i - tv->left_pos] : 'N';
70 mvaddch(1, tv->ccol++, c);
72 if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1);
74 call = bam_maqcns_call(n, pl, tv->bmc);
76 c = ",ACMGRSVTWYHKDBN"[call>>28&0xf];
77 i = (call>>8&0xff)/10+1;
79 attr |= COLOR_PAIR(i);
80 if (c == toupper(rb)) c = '.';
82 mvaddch(2, tv->ccol, c);
85 // calculate maximum insert
86 for (i = 0; i < n; ++i) {
87 const bam_pileup1_t *p = pl + i;
88 if (p->indel > 0 && max_ins < p->indel) max_ins = p->indel;
92 for (j = 0; j <= max_ins; ++j) {
93 for (i = 0; i < n; ++i) {
94 const bam_pileup1_t *p = pl + i;
95 int row = TV_MIN_ALNROW + p->level - tv->row_shift;
98 if (tv->base_for == TV_BASE_COLOR_SPACE &&
99 (c = bam_aux_getCSi(p->b, p->qpos))) {
100 c = bam_aux_getCSi(p->b, p->qpos);
101 // assume that if we found one color, we will be able to get the color error
102 if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos)) c = bam1_strand(p->b)? ',' : '.';
105 c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos)];
106 if (tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
110 if (j > p->indel) c = '*';
112 if (tv->base_for == TV_BASE_NUCL) {
113 c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos + j)];
114 if (j == 0 && tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.';
117 c = bam_aux_getCSi(p->b, p->qpos + j);
118 if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos + j)) c = bam1_strand(p->b)? ',' : '.';
122 if (row > TV_MIN_ALNROW && row < tv->mrow) {
125 if (((p->b->core.flag&BAM_FPAIRED) && !(p->b->core.flag&BAM_FPROPER_PAIR))
126 || (p->b->core.flag & BAM_FSECONDARY)) attr |= A_UNDERLINE;
127 if (tv->color_for == TV_COLOR_BASEQ) {
128 x = bam1_qual(p->b)[p->qpos]/10 + 1;
130 attr |= COLOR_PAIR(x);
131 } else if (tv->color_for == TV_COLOR_MAPQ) {
132 x = p->b->core.qual/10 + 1;
134 attr |= COLOR_PAIR(x);
135 } else if (tv->color_for == TV_COLOR_NUCL) {
136 x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)] + 5;
137 attr |= COLOR_PAIR(x);
138 } else if(tv->color_for == TV_COLOR_COL) {
140 switch(bam_aux_getCSi(p->b, p->qpos)) {
141 case '0': x = 0; break;
142 case '1': x = 1; break;
143 case '2': x = 2; break;
144 case '3': x = 3; break;
145 case '4': x = 4; break;
146 default: x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; break;
149 attr |= COLOR_PAIR(x);
150 } else if(tv->color_for == TV_COLOR_COLQ) {
151 x = bam_aux_getCQi(p->b, p->qpos);
152 if(0 == x) x = bam1_qual(p->b)[p->qpos];
155 attr |= COLOR_PAIR(x);
158 mvaddch(row, tv->ccol, bam1_strand(p->b)? tolower(c) : toupper(c));
164 attr = COLOR_PAIR(8);
166 mvaddch(1, tv->ccol++, c);
168 } else mvaddch(1, tv->ccol++, c);
174 tview_t *tv_init(const char *fn, const char *fn_fa)
176 tview_t *tv = (tview_t*)calloc(1, sizeof(tview_t));
178 tv->idx = bam_index_load(fn);
179 if (tv->idx == 0) exit(1);
180 tv->fp = bam_open(fn, "r");
181 bgzf_set_cache_size(tv->fp, 8 * 1024 *1024);
183 tv->header = bam_header_read(tv->fp);
184 tv->lplbuf = bam_lplbuf_init(tv_pl_func, tv);
185 if (fn_fa) tv->fai = fai_load(fn_fa);
186 tv->bmc = bam_maqcns_init();
188 bam_maqcns_prepare(tv->bmc);
191 keypad(stdscr, TRUE);
195 tv->mrow = 24; tv->mcol = 80;
196 getmaxyx(stdscr, tv->mrow, tv->mcol);
197 tv->wgoto = newwin(3, TV_MAX_GOTO + 10, 10, 5);
198 tv->whelp = newwin(28, 40, 5, 5);
199 tv->color_for = TV_COLOR_MAPQ;
201 init_pair(1, COLOR_BLUE, COLOR_BLACK);
202 init_pair(2, COLOR_GREEN, COLOR_BLACK);
203 init_pair(3, COLOR_YELLOW, COLOR_BLACK);
204 init_pair(4, COLOR_WHITE, COLOR_BLACK);
205 init_pair(5, COLOR_GREEN, COLOR_BLACK);
206 init_pair(6, COLOR_CYAN, COLOR_BLACK);
207 init_pair(7, COLOR_YELLOW, COLOR_BLACK);
208 init_pair(8, COLOR_RED, COLOR_BLACK);
209 init_pair(9, COLOR_BLUE, COLOR_BLACK);
213 void tv_destroy(tview_t *tv)
215 delwin(tv->wgoto); delwin(tv->whelp);
218 bam_lplbuf_destroy(tv->lplbuf);
219 bam_maqcns_destroy(tv->bmc);
220 bam_index_destroy(tv->idx);
221 if (tv->fai) fai_destroy(tv->fai);
223 bam_header_destroy(tv->header);
228 int tv_fetch_func(const bam1_t *b, void *data)
230 tview_t *tv = (tview_t*)data;
232 uint32_t *cigar = bam1_cigar(b); // this is cheating...
234 for (i = 0; i <b->core.n_cigar; ++i) {
235 if ((cigar[i]&0xf) == BAM_CREF_SKIP)
236 cigar[i] = cigar[i]>>4<<4 | BAM_CDEL;
239 bam_lplbuf_push(b, tv->lplbuf);
243 int tv_draw_aln(tview_t *tv, int tid, int pos)
247 tv->curr_tid = tid; tv->left_pos = pos;
248 tv->last_pos = tv->left_pos - 1;
250 // print ref and consensus
253 if (tv->ref) free(tv->ref);
254 str = (char*)calloc(strlen(tv->header->target_name[tv->curr_tid]) + 30, 1);
255 sprintf(str, "%s:%d-%d", tv->header->target_name[tv->curr_tid], tv->left_pos + 1, tv->left_pos + tv->mcol);
256 tv->ref = fai_fetch(tv->fai, str, &tv->l_ref);
260 bam_lplbuf_reset(tv->lplbuf);
261 bam_fetch(tv->fp, tv->idx, tv->curr_tid, tv->left_pos, tv->left_pos + tv->mcol, tv, tv_fetch_func);
262 bam_lplbuf_push(0, tv->lplbuf);
264 while (tv->ccol < tv->mcol) {
265 int pos = tv->last_pos + 1;
266 if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1);
267 mvaddch(1, tv->ccol++, (tv->ref && pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N');
273 static void tv_win_goto(tview_t *tv, int *tid, int *pos)
277 wborder(tv->wgoto, '|', '|', '-', '-', '+', '+', '+', '+');
278 mvwprintw(tv->wgoto, 1, 2, "Goto: ");
280 int c = wgetch(tv->wgoto);
282 if (c == KEY_BACKSPACE || c == '\010' || c == '\177') {
284 } else if (c == KEY_ENTER || c == '\012' || c == '\015') {
285 int _tid = -1, _beg, _end;
286 bam_parse_region(tv->header, str, &_tid, &_beg, &_end);
288 *tid = _tid; *pos = _beg;
291 } else if (isgraph(c)) {
292 if (l < TV_MAX_GOTO) str[l++] = c;
293 } else if (c == '\027') l = 0;
294 else if (c == '\033') return;
296 for (i = 0; i < TV_MAX_GOTO; ++i) mvwaddch(tv->wgoto, 1, 8 + i, ' ');
297 mvwprintw(tv->wgoto, 1, 8, "%s", str);
301 static void tv_win_help(tview_t *tv) {
303 WINDOW *win = tv->whelp;
304 wborder(win, '|', '|', '-', '-', '+', '+', '+', '+');
305 mvwprintw(win, r++, 2, " -=- Help -=- ");
307 mvwprintw(win, r++, 2, "? This window");
308 mvwprintw(win, r++, 2, "Arrows Small scroll movement");
309 mvwprintw(win, r++, 2, "h,j,k,l Small scroll movement");
310 mvwprintw(win, r++, 2, "H,J,K,L Large scroll movement");
311 mvwprintw(win, r++, 2, "ctrl-H Scroll 1k left");
312 mvwprintw(win, r++, 2, "ctrl-L Scroll 1k right");
313 mvwprintw(win, r++, 2, "space Scroll one screen");
314 mvwprintw(win, r++, 2, "backspace Scroll back one screen");
315 mvwprintw(win, r++, 2, "g Go to specific location");
316 mvwprintw(win, r++, 2, "m Color for mapping qual");
317 mvwprintw(win, r++, 2, "n Color for nucleotide");
318 mvwprintw(win, r++, 2, "b Color for base quality");
319 mvwprintw(win, r++, 2, "c Color for cs color");
320 mvwprintw(win, r++, 2, "z Color for cs qual");
321 mvwprintw(win, r++, 2, ". Toggle on/off dot view");
322 mvwprintw(win, r++, 2, "s Toggle on/off ref skip");
323 mvwprintw(win, r++, 2, "N Turn on nt view");
324 mvwprintw(win, r++, 2, "C Turn on cs view");
325 mvwprintw(win, r++, 2, "i Toggle on/off ins");
326 mvwprintw(win, r++, 2, "q Exit");
328 mvwprintw(win, r++, 2, "Underline: Secondary or orphan");
329 mvwprintw(win, r++, 2, "Blue: 0-9 Green: 10-19");
330 mvwprintw(win, r++, 2, "Yellow: 20-29 White: >=30");
335 void tv_loop(tview_t *tv)
338 tid = tv->curr_tid; pos = tv->left_pos;
342 case '?': tv_win_help(tv); break;
344 case 'q': goto end_loop;
345 case 'g': tv_win_goto(tv, &tid, &pos); break;
346 case 'm': tv->color_for = TV_COLOR_MAPQ; break;
347 case 'b': tv->color_for = TV_COLOR_BASEQ; break;
348 case 'n': tv->color_for = TV_COLOR_NUCL; break;
349 case 'c': tv->color_for = TV_COLOR_COL; break;
350 case 'z': tv->color_for = TV_COLOR_COLQ; break;
351 case 's': tv->no_skip = !tv->no_skip; break;
353 case 'h': --pos; break;
355 case 'l': ++pos; break;
357 case 'H': pos -= 20; break;
359 case 'L': pos += 20; break;
360 case '.': tv->is_dot = !tv->is_dot; break;
361 case 'N': tv->base_for = TV_BASE_NUCL; break;
362 case 'C': tv->base_for = TV_BASE_COLOR_SPACE; break;
363 case 'i': tv->ins = !tv->ins; break;
364 case '\010': pos -= 1000; break;
365 case '\014': pos += 1000; break;
366 case ' ': pos += tv->mcol; break;
368 case 'j': --tv->row_shift; break;
370 case 'k': ++tv->row_shift; break;
372 case '\177': pos -= tv->mcol; break;
373 case KEY_RESIZE: getmaxyx(stdscr, tv->mrow, tv->mcol); break;
376 if (pos < 0) pos = 0;
377 if (tv->row_shift < 0) tv->row_shift = 0;
378 tv_draw_aln(tv, tid, pos);
384 int bam_tview_main(int argc, char *argv[])
388 fprintf(stderr, "Usage: bamtk tview <aln.bam> [ref.fasta]\n");
391 tv = tv_init(argv[1], (argc == 2)? 0 : argv[2]);
392 tv_draw_aln(tv, 0, 0);
397 #else // #ifdef _HAVE_CURSES
399 #warning "No curses library is available; tview is disabled."
400 int bam_tview_main(int argc, char *argv[])
402 fprintf(stderr, "[bam_tview_main] The ncurses library is unavailable; tview is not compiled.\n");
405 #endif // #ifdef _HAVE_CURSES