static void unpad_seq(bam1_t *b, kstring_t *s)
{
int k, j, i;
+ int length;
uint32_t *cigar = bam1_cigar(b);
uint8_t *seq = bam1_seq(b);
- ks_resize(s, b->core.l_qseq);
+ // b->core.l_qseq gives length of the SEQ entry (including soft clips, S)
+ // We need the padded length after alignment from the CIGAR (excluding
+ // soft clips S, but including pads)
+ length = 0;
+ for (k = 0; k < b->core.n_cigar; ++k) {
+ int op, ol;
+ op= bam_cigar_op(cigar[k]);
+ ol = bam_cigar_oplen(cigar[k]);
+ if (op == BAM_CMATCH || op == BAM_CEQUAL || op == BAM_CDIFF || op == BAM_CDEL || op == BAM_CPAD)
+ length += ol;
+ }
+ ks_resize(s, length);
for (k = 0, s->l = 0, j = 0; k < b->core.n_cigar; ++k) {
int op, ol;
op = bam_cigar_op(cigar[k]);
assert(-1);
}
}
+ assert(length == s->l);
}
int bam_pad2unpad(bamFile in, bamFile out)