static void unpad_seq(bam1_t *b, kstring_t *s)
{
int k, j, i;
+ int length;
uint32_t *cigar = bam1_cigar(b);
uint8_t *seq = bam1_seq(b);
- ks_resize(s, b->core.l_qseq);
+ // b->core.l_qseq gives length of the SEQ entry (including soft clips, S)
+ // We need the padded length after alignment from the CIGAR (excluding
+ // soft clips S, but including pads from CIGAR D operations)
+ length = 0;
+ for (k = 0; k < b->core.n_cigar; ++k) {
+ int op, ol;
+ op= bam_cigar_op(cigar[k]);
+ ol = bam_cigar_oplen(cigar[k]);
+ if (op == BAM_CMATCH || op == BAM_CEQUAL || op == BAM_CDIFF || op == BAM_CDEL)
+ length += ol;
+ }
+ ks_resize(s, length);
for (k = 0, s->l = 0, j = 0; k < b->core.n_cigar; ++k) {
int op, ol;
op = bam_cigar_op(cigar[k]);
j += ol;
} else if (op == BAM_CHARD_CLIP) {
/* do nothing */
- } else if (op == BAM_CDEL || op == BAM_CPAD) {
+ } else if (op == BAM_CDEL) {
for (i = 0; i < ol; ++i) s->s[s->l++] = 0;
} else {
fprintf(stderr, "[depad] ERROR: Didn't expect CIGAR op %c in read %s\n", BAM_CIGAR_STR[op], bam1_qname(b));
assert(-1);
}
}
+ assert(length == s->l);
}
int bam_pad2unpad(bamFile in, bamFile out)
pre_op = bam_cigar_op(cigar2[i-2]);
post_op = bam_cigar_op(cigar2[i]);
/* Note don't need to check for X/= as code above will use M only */
- if ((pre_op == BAM_CMATCH || pre_op == BAM_CDIFF) || (post_op == BAM_CMATCH || post_op == BAM_CDIFF)) {
+ if ((pre_op == BAM_CMATCH || pre_op == BAM_CDEL) && (post_op == BAM_CMATCH || post_op == BAM_CDEL)) {
/* This is a redundant P operator */
cigar2[i-1] = 0; // i.e. 0M
/* If had same operator either side, combine them in post_op */
int result=0;
if (argc == 1) {
fprintf(stderr, "Usage: samtools depad <in.bam>\n");
+ fprintf(stderr, "\nRequires embedded reference sequences (before the reads for that reference).\n");
return 1;
}
in = strcmp(argv[1], "-")? bam_open(argv[1], "r") : bam_dopen(fileno(stdin), "r");